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Showing sessions 1 - 10 of (21) TOTAL sessions
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Event : CYTO105


Session : CYTO1303
WK1: "You Reap What You Sow:" Addressing Pre-analytical Issues in Molecular Cytopathology
Workshop 1 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Mauro A. Saieg, MD, PhD , Gilda Da Cunha Santos, MD, PhD, FRCPC, FIAC
Download Format(s) :
VIDEO
$50.00


  • Description: This workshop will review methods for acquiring and preparing excellent aspirates in order to assure high quality material, sine qua non condition for a successful subsequent DNA/RNA extraction. A case discussion approach will be used to illustrate practical aspects of basic principles and application of different cytological preparations for a variety of molecular analysis. Presentations of the cases will include algorithms for sample triage for maximizing DNA/RNA retrieval. Application of cytological material to the current studies and their advantages, limitations and clinical relevance will also be emphasized. The workshop is designed for residents, pathologists and cytotechnologists with an interest in molecular pathology and looking for an update in recent advances in molecular profiling, emerging technologies as well as the basic requirements for successful results. Information presented will be of value to professionals who want to improve the quality of specimens being obtained/received in their laboratories for optimal morphological and molecular analysis.
  • Learning Objectives: 1.) To highlight the importance of techniques used for obtaining fresh material and excellent aspirates, cytospins and cell block preparations for molecular studies, including the importance of rapid on-site assessment, with emphasis on PCR based and cyt 2.) To review different techniques for storing cytological material for molecular analysis such as archival slides, cryopreservation and FTA cards and discuss their advantages, disadvantages and limitations 3.) To emphasize the basic principles and the current application of different molecular studies and their relevance for diagnosis, prognosis and for predicting specific treatments with special focus on targeted therapies 4.) To provide an overview of recent advances in molecular profiling of solid tumors acquired through advanced genomic technologies including next generation sequencing and array comparative genomic hybridization
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1304
WK2: Endoscopic Ultrasound-Guided Fine Needle Aspiration: Patient Centered Personalized Medicine
Workshop 2 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Darshana N. Jhala, MD, BMus , Nirag C. Jhala, MD, MIAC , Ramesh Shah, DMD
Download Format(s) :
VIDEO
$50.00


  • Description: A patient with pancreatic mass in this day and age is more informed then ever before. They are usually informed about the procedure and come with many expectations. They need an accurate diagnosis so that they can benefit from making an informed management decisions. Endoscopic ultrasound-guided fine needle aspiration from pancreas as well as mediastinum have revolutionized how a diagnosis of pancreatic cancer as well as lung cancer staging is made. They, however, pose unique set of challenges for cytotechnologists and cytopathologists. A solid algorithmic approach to these lesions not only will help to arrive at an accurate diagnosis but also help procure samples for molecular studies that will help guide additional therapy. This course will highlight an algorithmic approach to select solid lesions for the pancreas as well as mediastinum as well as provide a solid road map to tackle some unusual lesions accessed utilizing EUS route.
  • Learning Objectives: 1.) To characterize what patients want from cytopathology team 2.) To demonstrate approach to solid pancreatic lesion 3.) To demonstrate approach to mediastinal lesions and utilization of ancillary studies
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1307
PL1: Personalized Genomic Medicine & Bioinformatics Changing Today's Practice of Cytopathology Sponsored by the Papanicolaou Society of Cytopathology
Panel Luncheon Seminar 1 (1.75 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Anna Berry, MD , Sara E. Monaco, MD , Liron Pantanowitz, MD, MIAC , Matthew A. Zarka, MD
Download Format(s) :
VIDEO
$50.00


  • Description: Next Generation Sequencing (NGS) techniques are the new phase in the evolution of cancer genomics. This technology will revolutionize our personalized approach to the treatment of cancer and the practice of clinical medicine. These tests can now be offered at dramatically reduced cost. This means that routine whole genome sequencing of clinical samples is affordable and a reality. However, challenges associated with pre-analytic issues (appropriate sample collection), analysis (data generation and manipulation) and post-analytic needs (data storage, reporting and mining) need to be addressed and dealt with. NGS and related technologies present an excellent opportunity for today's cytopathologists to be at the leading edge of personalized medicine. Cytologists need to be aware of these rapid advances in the field of personalized genomic medicine and what this signifies for their limited cytology samples and the clinical practice of Cytopathology. The panel speakers will address the key factors related to cytology samples, review the fundamentals of this molecular technology, and discuss the informatics needs and computational tools required for the successful implementation of NGS.
  • Learning Objectives: 1.) Highlight key factors related to cytology sample collection and processing for molecular testing 2.) Review the fundamentals of molecular and next generation sequencing (NGS) technology 3.) Discuss the informatics needs and computational tools required for NGS in clinical practice
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1310
WK3: Headaches and Highlights of Hepatic and Renal FNA
Workshop 3 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Gladwyn Leiman, MBBCh, FIAC, FRCPath
Download Format(s) :
VIDEO
$50.00


  • Description: In this session, the basics of both liver and renal cytopathology will be presented in relation to clinical indications and in particular, therapeutic options available at this time. In addition to usual morphological features, occasional examples of more difficult cytologic presentations will be shown. Benign and malignant entities will be shown, with appropriate differential diagnoses. Adjunctive studies specific for these sites will be discussed.
  • Learning Objectives: 1.) Participants will appreciate the full spectrum of benign and malignant lesions of the liver, including indications, complications, morphology and differential diagnosis. Common and uncommon presentations will be shown 2.) In terms of the renal procedures, participants will hear and be able to collate the many imaging, surgical, and minimally invasive therapies introduced for renal tumors over the last decade 3.) Renal Cytopathology will be presented as an integral part of the new approach to renal lesions, with special reference to those benign and malignant lesions not requiring full nephrectomy 4.) In both sites, current immunological and molecular techniques appropriate to the location, will be made available
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1312
WK4: Urine Cytology - When to FISH
Workshop 4 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Oscar Lin, MD, PhD , Dorota E. Rudomina, CT(ASCP)
Download Format(s) :
VIDEO
$50.00


  • Description: Urine cytology is a commonly used test with known limitations, which have been used in association or have been replaced by FISH in certain practices. The purpose of the presentation is to discuss the criteria and terminology currently used in urine cytology including its limitations. The presentations will also discuss the advantages and limitations of FISH analysis in urine specimens.
  • Learning Objectives: 1.) Present the current morphologic criteria and terminology for urine cytology 2.) Present the rationale behind the FISH analysis in urine specimens, including data on indications, sensitivity and specificity and pitfalls 3.) Discuss effective ways to use FISH clinical practice
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1313
WK5: A Practical Approach to FNA Biopsy of Reactive versus Lymphoproliferative Disorders with Emphasis on Ancillary/Molecular Techniques
Workshops 5 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Michael W. Beaty, MD , Simon Bergman, MD
Download Format(s) :
VIDEO
$50.00


  • Description: Fine needle aspiration (FNA) biopsy of lymph nodes is the mainstay is often the first step in the diagnosis of primary lymphoproliferative disorders. FNA is being increasingly accepted by most patients and clinicians as a non-invasive method for evaluating lymphadenopathy, and is particularly suited for deep-seated lymph nodes. FNA of lymph nodes has high sensitivity and specificity in distinguishing between benign and malignant lesions. The purpose of this course is to present FNA biopsy of lymphoproliferative lesions and correlative results of ancillary techniques, especially immunophenotyping, in this vast array of reactive and neoplastic entities. In the context of clinical presentations, the aspiration cytomorphologic features of benign and malignant lymphoid disorders will be detailed. The former will include reactive follicular hyperplasia, infectious mononucleosis, Castleman's disease, granulomatous inflammation, Rosai-Dorfman disease, and suppurative lymphadenitis. Both nodal and extranodal neoplastic lymphoproliferative disorders will be presented, Hodgkin lymphoma and the non-Hodgkin lymphomas, from the perspective of the current WHO classification. Correlation with histopathology will be done when relevant. The often-crucial role of immunophenotyping of aspirated material by flow cytometry will be presented including a discussion of both the basics and the necessary details.
  • Learning Objectives: 1.) Distinguish and diagnose fine needle aspiration biopsies of benign/reactive nodal lymphoproliferative processes 2.) Distinguish and diagnose fine needle aspiration biopsies of nodal and extranodal malignant lymphoproliferative disorders 3.) Discuss morphologic and phenotypic limitations of FNA biopsies, and recognize indications, advantages/disadvantages, pitfalls and limitations of FNA biopsy of lymphoproliferative lesions 4.) Integrate cytomorphology with ancillary immunophenotypic and molecular analyses to render more specific interpretations 5.) Practice a multi-modality diagnostic approach to FNA biopsies of nodal lymphoproliferative disorders
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1314
WK6: The Challenge of Ensuring Adequacy in the New Era of Targeted Lung Cancer Therapy
Workshops 6 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : John M. Stewart, MD, PhD , Michael J. Thrall, MD
Download Format(s) :
VIDEO
$50.00


  • Description: In recent years, therapeutic options for lung cancer have markedly expanded, with numerous additional targeted drugs currently in the development pipeline. While this offers new hope for patients, it also places an increasing burden on cytologists who now need to provide more information about the morphology of the carcinoma they are examining while preserving cells for vitally important molecular ancillary testing. The goal of this session is to review cytology material in association with corresponding small biopsies of lung carcinoma. Special emphasis will be placed on emerging diagnostic issues and related problems of ensuring "adequacy." In the past the main job of the cytologist was just to confirm malignancy and separate small cell carcinoma from non-small cell carcinoma. Usually only a relatively small number of cells and just a single good quality slide would suffice for this limited role. Now this is just the beginning of an ever-more complicated diagnostic algorithm. In many cases immunocytochemistry is now essential, hinging on the ability of the cytology laboratory to perform staining on smears or consistently create good cell blocks. Tips for doing both will be part of the session. In the not-so-distant past, immunocytochemistry algorithms mostly focused on maximizing diagnostic certainty, often resulting in panels of four or more markers to separate adenocarcinoma from squamous cell carcinoma. Now, however, preservation of material for genetic testing is at least as important as subtyping, leading to new recommendations to only use two markers. The most commonly used two-marker panel, p63 and TTF-1, can be difficult to interpret and the implications of various staining patterns, as well as optimal ways to proceed, will be discussed. Depending on the morphology and immunocytochemistry, additional molecular studies including PCR for EGFR and FISH for ALK, may also be needed. The session will briefly review the clinical logic behind all of this testing and the increasingly subtle interaction between morphology and treatment, including the correlation with certain adenocarcinoma subtypes with EGFR and ALK mutations. The various cytology materials that can be used for molecular testing, along with pros and cons of different approaches, such as the use of alcohol-fixed smears as opposed to formalin-fixed cell blocks, will also be elaborated upon, especially with regard to how cytologists can "stretch" limited material so as to maximize the success of all testing modalities and thereby avoid repeat testing and the associated delays. As oncologists and surgeons push for ever-more specific diagnoses, there is a growing burden on cytologists not just to perform immediate adequacy but to assure that sufficient cells are present for all possible testing. The demand for more material is accelerating the trend toward increasing numbers of CT-guided core biopsies of lung tumors. The cytology of touch preparations, including how it differs from conventional FNA material and pitfalls in correlation with the corresponding biopsy, will be reviewed. EBUS-guided FNA of lung tumors and mediastinal nodes has also become more main stream in recent years as a less invasive means of sampling tumors. A review of this technology and the corresponding cytology challenges, particularly the resultant increase in mediastinal lymph node analysis, will also be included in the session.
  • Learning Objectives: 1.) Review the underlying factors that are changing demands on cytologists who perform immediate adequacy on lung specimens in the new era of targeted therapies and molecular testing 2.) Demonstrate practical approaches for achieving suitable diagnoses while preserving cellular material for additional ancillary studies needed for targeted therapies 3.) Correlate cytologic findings from touch preparation with corresponding small biopsies from the lung, with an emphasis on how touch preparations differ from FNA and on pitfalls in adequacy assessment and interpretation 4.) Compare and contrast endoscopic ultrasound-guided FNA material with material gathered percutaneously by CT guidance
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1317
WK7: The ‘Elusive' HSIL: Analyses of Liquid-Based Pap Test Immediately Preceding Histologically- Proven HSIL
Workshop 7 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Susan A. Alperstein, MS, CT(ASCP) , Rana S. Hoda, MD, FIAC, MBBS
Download Format(s) :
MP3
$50.00


  • Description: Correlation of biopsy-proven high grade squamous intraepithelial lesion (HSIL) with the immediately preceding Pap test diagnosis is not a required quality assurance (QA) monitor for cytology and is therefore not performed. Moreover, literature on this subject is also scarce. In a large landmark study, performed on conventional Pap smears, 68.6% of Pap smears preceding a histologically-proven HSIL only showed a minimal abnormality (Kinny WK et al. Obstet Gynecol; 1998; 91:973). In a recent College of American Pathologist's 4-year review of Gyn Cytology Proficiency Test (PT) failures revealed that, around half of errors for pathologists failing PAP PT (43.3%) are automatic failure, i.e. misdiagnosing category D: HSIL or Ca as category B (negative) (Ducatman, Arch Pathol Lab Med 2011; 135:1442). In this practical case-based course, the experienced faculty will review all liquid-based Pap test cytologic diagnoses immediately preceding histologic HSIL and will examine causes for discrepancy. Utility of image-based automated screening devices such as ThinPrep Imaging System and automated devices for SurePath in the detection of HSIL will also be demonstrated. Utility of high risk (hr) HPV test in the subsequent management of patients based on ASCCP guidelines will be addressed. Emphasis will be on various cytologic presentations of HSIL on LBP and its pitfalls.
  • Learning Objectives: 1.) Understand various cytomorphological patterns of HSIL on liquid-based preparations (LBP) 2.) Recognize the importance of correlating biopsy-proven HSIL with the immediately preceding Pap test diagnosis 3.) Recognize the utility of automated image-based screening systems for LBP on HSIL detection 4.) Understand the impact of your diagnosis and HPV test on patient management
  • AUDIO ONLY
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1318
PL2: Cervical Cancer Screening in Australia, The Past, Present and Future: The Baby in the Bath Water
Panel Luncheon 2 (1.75 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Syed Z. Ali, MD , Annabelle Farnsworth, MB, BS, FRCPA, FIAC, DipCytopath (RCPA) , Andrew Field, MB BS(Hons), FRCPA, FIAC, DipCytopath(RCPA) , Marion Saville, MB ChB AmBd, (Anat Path&Cytopath), FIAC Grad Dip M
Download Format(s) :
VIDEO
$50.00


  • Description: In Australia there is a nationally organized and publicly funded cervical cancer screening service based on the cervical Pap test that has reduced cervical cancer rates and mortality greatly over the last twenty years. Integrating HPV testing into this cancer screening service is underway in an environment where Australia has instituted free, publicly funded vaccination of all school girls and young women in various age cohorts since 2007, and now in 2013, young school age boys. The various arguments for HPV testing introduction in this setting will be presented after a brief overview of the Australian program's history and development and a current review. The impact of the vaccination program will be presented, and the way forward for the cervical cancer screening program discussed.
  • Learning Objectives: 1.) Understand the impacts of HPV vaccination and HPV testing on an established, best practice national, free cervical cancer screening service based on the cervical Pap 2.) Appreciate the need for careful planning to integrate HPV testing in a scientifically based and cost effective manner to impact on cervical cancer screening 3.) Appreciate the need to assess each country's current screening programs and cervical cytology services before introducing HPV testing
  • Click the PDF icon to claim your CME/CMLE/SAM credits


Session : CYTO1320
WK8: Morphology and Molecular Update in Thoracic and Mediastinal Cytopathology
Workshop 8 (1.5 CME/CMLE/SAM)
Conference : Purchase Podcasts Individually (2013)
Speaker(s) : Sara E. Monaco, MD , Liron Pantanowitz, MD, MIAC
Download Format(s) :
VIDEO
$50.00


  • Description: This session will provide a comprehensive review of challenging lesions that can be seen in the thoracic cavity, including mediastinal lymph nodes, soft tissue, lung, and pleura. After a brief introduction and didactic presentation, a case-based approach will be utilized to highlight interesting cases, including challenging mesotheliomas, endobronchial ultrasound guided FNA (EBUS-FNA) cases, and other image-guided FNAs of the lung and mediastinum. The discussion will focus on the cytomorphology, differential diagnosis, and diagnostic pitfalls. In addition, there will be a focus on discussing the ancillary studies and updating the audience on how we utilize these tests in our practice as well as present salient points of the current literature in this area of cytopathology. We will emphasize how fluorescence in-situ hybridization (FISH) and molecular techniques can be applied to thoracic cytological specimens. The cases will highlight common diagnostic dilemmas, including mesothelioma and its variants, non-small cell carcinomas of the lung, neuroendocrine tumors, thymic tumors, spindle cell lesions, and lymphoid lesions.
  • Learning Objectives: 1.) Review the cytomorphology and diagnostic challenges in cytological specimens from thoracic lesions including the lung, lymph nodes, thymus, mediastinal soft tissue, and pleura 2.) Understand the various cytological patterns for lesions in the mediastinum including epithelioid, lymphoid, and spindle cell lesions, and how this can help one to formulate a differential diagnosis 3.) Recognize and understand how ancillary studies such as FISH and molecular techniques can be applied in thoracic and mediastinal cytopathology
  • Click the PDF icon to claim your CME/CMLE/SAM credits



     


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